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histamine release buffer  (Immunotec inc)

 
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    Immunotec inc histamine release buffer
    Histamine Release Buffer, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histamine+release+buffer/histamine+release+buffer/pm34731787-102-15-18
    Average 90 stars, based on 1 article reviews
    histamine release buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Cell surface membrane antigen phenotype of human gastrointestinal mast cells.
    Article Snippet: Background: Mast cells (MC) are important effector cells of allergic and infl ammatory reactions in diverse organs.. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinfl ammatory mediators and cytokines.. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.

    Incubation:

    Article Title: Cell surface membrane antigen phenotype of human gastrointestinal mast cells.
    Article Snippet: Background: Mast cells (MC) are important effector cells of allergic and infl ammatory reactions in diverse organs.. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinfl ammatory mediators and cytokines.. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.

    Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis.
    Article Snippet: .. Dextrannriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) ere incubated in histamine release buffer (Immunotech, Marseille, France) n the absence or presence of polyclonal anti-IgE antibody (0.01 −10 μg/ml) t 37 °C for 2 h. After incubation, cells were centrifuged at 4 °C, and the ell-free supernatants and total suspensions were analyzed for histamine ontent by radioimmunoassay (Immunotech). ..

    Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis
    Article Snippet: .. Dextran-enriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) were incubated in histamine release buffer (Immunotech, Marseille, France) in the absence or presence of polyclonal anti-IgE antibody (0.01−10 μg/ml) at 37°C for 2 h. After incubation, cells were centrifuged at 4°C, and the cell-free supernatants and total suspensions were analyzed for histamine content by radioimmunoassay (Immunotech). ..

    Article Title: Effects of various statins on cytokine-dependent growth and IgE-dependent release of histamine in human mast cells.
    Article Snippet: Mast cells (MCs) are multifunctional effector cells of the immune system (1, 2).. These cells produce an array of biologic mediators and play a crucial role in allergic and inflammatory reactions (1–3).. MCs also express a number of critical cell surface antigens including cytokine receptors and the high affinity receptor for immunoglobulin E (IgE; FceRI; 4, 5).

    Article Title: Effects of ibrutinib on proliferation and histamine release in canine neoplastic mast cells.
    Article Snippet: .. After incubation, cells were washed in phosphate-buffered saline (PBS, Gibco Life Technologies), resuspended in histamine release buffer (HRB, Immunotech, Marseille, France) and then incubated with 5 μg/mL goat anti-dog-IgE antibody (Bethyl Laboratories) at 37 C for 30 minutes. .. After incubation, cells were centrifuged at 4 C, and the cell-free supernatants and cell pellets were recovered and analysed for histamine content by radioimmunoassay (RIA, Immunotech).

    Control:

    Article Title: Cell surface membrane antigen phenotype of human gastrointestinal mast cells.
    Article Snippet: Background: Mast cells (MC) are important effector cells of allergic and infl ammatory reactions in diverse organs.. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinfl ammatory mediators and cytokines.. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.

    RIA Assay:

    Article Title: Cell surface membrane antigen phenotype of human gastrointestinal mast cells.
    Article Snippet: Background: Mast cells (MC) are important effector cells of allergic and infl ammatory reactions in diverse organs.. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinfl ammatory mediators and cytokines.. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.

    Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis.
    Article Snippet: .. Dextrannriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) ere incubated in histamine release buffer (Immunotech, Marseille, France) n the absence or presence of polyclonal anti-IgE antibody (0.01 −10 μg/ml) t 37 °C for 2 h. After incubation, cells were centrifuged at 4 °C, and the ell-free supernatants and total suspensions were analyzed for histamine ontent by radioimmunoassay (Immunotech). ..

    Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis
    Article Snippet: .. Dextran-enriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) were incubated in histamine release buffer (Immunotech, Marseille, France) in the absence or presence of polyclonal anti-IgE antibody (0.01−10 μg/ml) at 37°C for 2 h. After incubation, cells were centrifuged at 4°C, and the cell-free supernatants and total suspensions were analyzed for histamine content by radioimmunoassay (Immunotech). ..

    Sedimentation:

    Article Title: Gastro-duodenal digestion products of the major peanut allergen Ara h 1 retain an allergenic potential.
    Article Snippet: Gastro-duodenal digestion products of the major peanut allergen Ara h 1 retain an allergenic potential T. Eiwegger , N. Rigby, L. Mondouletz, H. Bernardz, M.-T. Krauth, A. Boehm, E. Dehlink , P. Valent, J. M. Walz, E. N. C. Mills and Z. Szépfalusi Department of Pediatrics, Division of General Pediatrics, Medical University of Vienna, Vienna, Austria, Institute of Food Research, Norwich Research Park, Norwich, UK, zLaboratoire d’Immuno-Allergie Alimentaire, INRA-CEA Saclay, France and Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria

    Saline:

    Article Title: Effects of ibrutinib on proliferation and histamine release in canine neoplastic mast cells.
    Article Snippet: .. After incubation, cells were washed in phosphate-buffered saline (PBS, Gibco Life Technologies), resuspended in histamine release buffer (HRB, Immunotech, Marseille, France) and then incubated with 5 μg/mL goat anti-dog-IgE antibody (Bethyl Laboratories) at 37 C for 30 minutes. .. After incubation, cells were centrifuged at 4 C, and the cell-free supernatants and cell pellets were recovered and analysed for histamine content by radioimmunoassay (RIA, Immunotech).



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    histamine release buffer  (Immunotec inc)
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    Immunotec inc histamine release buffer
    Histamine Release Buffer, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histamine+release+buffer/histamine+release+buffer/pm34731787-102-15-18
    Average 90 stars, based on 1 article reviews
    histamine release buffer - by Bioz Stars, 2026-09
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    histamine release buffer hrb  (Immunotec inc)
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    Immunotec inc histamine release buffer hrb
    Histamine Release Buffer Hrb, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histamine+release+buffer/histamine+release+buffer+hrb/pmc06900099-66-14-18
    Average 90 stars, based on 1 article reviews
    histamine release buffer hrb - by Bioz Stars, 2026-09
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    histamine release buffer (hrb)  (Immunotec inc)
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    Immunotec inc histamine release buffer (hrb)
    Effects of ibrutinib on <t>IgE‐mediated</t> <t>histamine</t> release in human basophils. Basophils ( BA ) obtained from three nonallergic donors (A) or patients allergic to Der p 2 (n=3) or Phl p 5 (n=3) (B) were preincubated in control medium (Co) or various concentrations of ibrutinib (0.001‐1 μmol/L) at 37°C for 30 minutes. Then, cells were exposed to anti‐IgE (1 μg/mL; nonallergic donors) or recombinant allergens (1 μg/mL of rDer p 2 or rPhl p 5; allergic patients) at 37°C for 30 minutes. After centrifugation, histamine concentrations were determined in supernatants and cell lysates. Histamine release is expressed as percentage of total histamine. Results show the percentage of control and represent mean± SD of three independent experiments. Asterisk (*): P <0.05 by Student's t test. (C) BA from two patients allergic to Der p 2 (left and right panel) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Then, cells were incubated in histamine release buffer ( <t>HRB</t> ) in the absence or presence of rDer p 2 (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. (D) Left panel: BA from a patient with chronic lymphocytic leukemia ( CLL ) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Thereafter, cells were incubated in HRB in the absence or presence of anti‐IgE (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. Right panel: In the same patient with CLL , BA were obtained before therapy with ibrutinib (pretreatment; ■‐■) and 14 days after treatment with 280 mg/day ibrutinib (post‐treatment; ●‐●). Cells were incubated in HRB in the absence or presence of anti‐IgE antibody E‐124.2.8 (0.001‐10 μg/mL) for 30 minutes. Then, histamine release was measured as described above. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates.
    Histamine Release Buffer (Hrb), supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histamine+release+buffer/histamine+release+buffer/pmc05655929-24-0-12
    Average 90 stars, based on 1 article reviews
    histamine release buffer (hrb) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of ibrutinib on IgE‐mediated histamine release in human basophils. Basophils ( BA ) obtained from three nonallergic donors (A) or patients allergic to Der p 2 (n=3) or Phl p 5 (n=3) (B) were preincubated in control medium (Co) or various concentrations of ibrutinib (0.001‐1 μmol/L) at 37°C for 30 minutes. Then, cells were exposed to anti‐IgE (1 μg/mL; nonallergic donors) or recombinant allergens (1 μg/mL of rDer p 2 or rPhl p 5; allergic patients) at 37°C for 30 minutes. After centrifugation, histamine concentrations were determined in supernatants and cell lysates. Histamine release is expressed as percentage of total histamine. Results show the percentage of control and represent mean± SD of three independent experiments. Asterisk (*): P <0.05 by Student's t test. (C) BA from two patients allergic to Der p 2 (left and right panel) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Then, cells were incubated in histamine release buffer ( HRB ) in the absence or presence of rDer p 2 (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. (D) Left panel: BA from a patient with chronic lymphocytic leukemia ( CLL ) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Thereafter, cells were incubated in HRB in the absence or presence of anti‐IgE (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. Right panel: In the same patient with CLL , BA were obtained before therapy with ibrutinib (pretreatment; ■‐■) and 14 days after treatment with 280 mg/day ibrutinib (post‐treatment; ●‐●). Cells were incubated in HRB in the absence or presence of anti‐IgE antibody E‐124.2.8 (0.001‐10 μg/mL) for 30 minutes. Then, histamine release was measured as described above. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates.

    Journal: Allergy

    Article Title: BTK inhibition is a potent approach to block IgE‐mediated histamine release in human basophils

    doi: 10.1111/all.13166

    Figure Lengend Snippet: Effects of ibrutinib on IgE‐mediated histamine release in human basophils. Basophils ( BA ) obtained from three nonallergic donors (A) or patients allergic to Der p 2 (n=3) or Phl p 5 (n=3) (B) were preincubated in control medium (Co) or various concentrations of ibrutinib (0.001‐1 μmol/L) at 37°C for 30 minutes. Then, cells were exposed to anti‐IgE (1 μg/mL; nonallergic donors) or recombinant allergens (1 μg/mL of rDer p 2 or rPhl p 5; allergic patients) at 37°C for 30 minutes. After centrifugation, histamine concentrations were determined in supernatants and cell lysates. Histamine release is expressed as percentage of total histamine. Results show the percentage of control and represent mean± SD of three independent experiments. Asterisk (*): P <0.05 by Student's t test. (C) BA from two patients allergic to Der p 2 (left and right panel) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Then, cells were incubated in histamine release buffer ( HRB ) in the absence or presence of rDer p 2 (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. (D) Left panel: BA from a patient with chronic lymphocytic leukemia ( CLL ) were incubated in control medium (Co) or 1 μmol/L ibrutinib at 37°C for 30 minutes. Thereafter, cells were incubated in HRB in the absence or presence of anti‐IgE (0.001‐10 μg/mL) at 37°C for 30 minutes. After incubation, cells were centrifuged at 4°C and cell‐free supernatants and cell suspensions analyzed for histamine content. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates. Right panel: In the same patient with CLL , BA were obtained before therapy with ibrutinib (pretreatment; ■‐■) and 14 days after treatment with 280 mg/day ibrutinib (post‐treatment; ●‐●). Cells were incubated in HRB in the absence or presence of anti‐IgE antibody E‐124.2.8 (0.001‐10 μg/mL) for 30 minutes. Then, histamine release was measured as described above. Histamine release is expressed as percentage of total histamine. Results represent the mean± SD of triplicates.

    Article Snippet: Histamine release buffer (HRB) and histamine radioimmunoassay (RIA) kit were purchased from Immunotech, and RPMI 1640 medium, Iscove's modified Dulbecco's medium (IMDM), and fetal calf serum (FCS) from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Control, Recombinant, Centrifugation, Incubation